Sterilization of glassware and apparatus used in microbiology. Autoclave is the nucleus of a microbiology laboratory. The autoclave is a large and complex machine that uses pressurized. Sks science offers several models of affordable yet highquality lab equipment items that could be useful in a microbiology lab, including digital pipette controllers and magnetic stirrers. This procedure is applicable for cleaning of all glassware, which is used for microbiological analysis in microbiology laboratory of quality control department 3. An assignment of microbiology laboratory use of microbiology lab, chemical and equipment book october 2008 with 17,365 reads how we measure reads. Glassware should be washed with soap and water, then rinsed with distilled water. Sterilization method aims at preserving the substance for a long time. The following standard and special practices, safety equipment and facilities apply to agents assigned to biosafety level 1.
Sop for cleaning of glassware used for microbiological testing. The cleaning of glassware is an important preliminary step prior to sterilisation in the microbiology. In addition to microorganisms, there are some chemicals used in this laboratory that are potentially harmful. This document describes the use of disinfectants for routine laboratory decontamination of surfaces and equipment. Students in a microbiology lab are provided plates of medium to use in their bacterial culturing. Laboratory personnel have specific training in the procedures conducted in the laboratory and are supervised by a scientist with general training in microbiology or a related science. Antiseptic a substance that inhibits the growth and development of microorganisms without killing them. Washing and cleaning of laboratory glassware general rules 1. Wash your hands with disinfectant soap when you arrive at the lab and again before you leave. Pdf an assignment of microbiology laboratory use of. In all instances, glassware must be physically and chemically clean and in many cases, it must be bacteriologically clean or sterile. This is a scientific laboratory course, and thus a working medical office floor plans pdf understanding of the. Microbiology laboratory safety rules and procedures. Laminar flow is used to maintain the clean environment.
This test is used to determine the ability of an organism to split tryptophan to form the compound indole. For this reason, the focus is on the use of a detergent. Glassware test tube used to mix, heat or store substances. June 2002 overview this microbiology laboratory manual is designed especially for the nonmajors, health science. It is used differentiate gram negative rods particularly e. Sop on decontamination and disposal of used media objective to lay down the procedure for decontamination and disposal of used media scope this sop is applicable for decontamination and disposal of used media. A modern microbiology laboratory should be furnished with the following equipment. Glassware used as laboratory apparatus offers a wide range of containment and transport functions for solutions and other liquids used in laboratories. Since the 1960s, numerous ingenious innovations have been introduced and used in clinical microbiology laboratories. Avessel,heatedafter evaporationhasalreadyoccurred,maycrack. It involves the application of heatchemical on the substance like drugs, food, surgical equipment, nutraceuticals etc. Are the correct reference cultures being used in the method and traceable to a recognised source. We do not imply these products are fit for any particular use.
U heck all glassware for damage as even small cracks, chips or scratches affect the strength of the c glassware. Autoclaving in microbiology laboratories the most widely used method for heat sterilization is autoclaving. As always you should test labware to ensure product compatibility. A variety of media are also required for specific uses. There are a number of lab ware for use made of different material. Usdafsis microbiology laboratory guidebook 3rd edition1998 i foreword the 1993 escherichia coli o157. The experiments introduce students to the activities of various organisms and the analyses used to study them. Sop for cleaning and sterilization of glassware used in microbiology laboratory standard operating procedure of cleaning and sterilization of glassware used for microbial analysis. Unlike static pdf laboratory experiments in microbiology 11th edition solution manuals or printed answer keys, our experts show you how to solve each problem stepbystep.
In the laboratory, bacteria are grown on nutrientrich substances called media. Essential microbiology lab equipment lab instruments. Cqi and pi seek to improve patient care by placing the emphasis s on not mistakes in the first place. The most important step is to keep the glassware after use in 3% lysolor any other disinfectant solution for disinfection. The keeping of a record of all such incidents is recommended. In microbiology lab, it is used for sterilizing inoculating loop, plating out cultures, transferring cultures, heatfixing of smears and creating a sterile zone for aseptic operation. Previously used or new glassware must be thoroughly cleaned. Any nonabrasive glassware detergent may be used for hand or automatic dishwasher cleaning. Fvms microbiology laboratory program faces significant challenges arising from new technologies being developed and used in food and feed, new methods of identifying and assessing microbial. Microbiology laboratory safety rules and procedure learn. Being chemically inert to the sample and reaction mixture taken. These include procedure manuals, maintenance schedules, calibrations, a quality assurance program, training and quality control. Different glasswares and labwares used in a microbiology.
As is common practice, clean all glassware before use. Autoclaving steam under pressure or pressure cooker. A number of glass wares and lab wares are required in the microbiology laboratory. The goal of the laboratory is to expose students to the wide variety of lives in the microbial world. Most laboratory glassware is manufactured with borosilicate glass, a particularly durable glass that can safely be used to hold chemicals being heated over a flame and to contain acidic or corrosive chemicals. Laboratory ultraviolet light sources princeton university. Standard operating procedure sop for laboratory disinfection issued. Instruments used in microbiology linkedin slideshare.
This equipment is used in microbiology laboratory for storing preserving cultures, media, and many sensitive materials fig. Bunsen burner bunsen burner is a common tool used in science lab fig. Microbiology module quality control in microbiology microbiology 148 notes tqm evolved as an activity to improve patient care by having the laboratory monitor its work to detect deficiency and subsequently correct them. Laboratory methods in microbiology is a laboratory manual based on the experience of the authors over several years in devising and organizing practical classes in microbiology to meet the requirements of students following courses in microbiology at the west of scotland agricultural college. For suspicion of tuberculosis use 1% sodium hypochlorite. Absolutely no food, drinks, chewing gum, or smoking is allowed in the laboratory. Glassware flask glassware used to heat and store substances. What are the different types of microscopy used in a. Glassware used in a chemistry laboratory is special. Because our labs and protocols involve not only living organisms, but also some that are potential pathogens, you must adhere to. Pages in category microbiology equipment the following 38 pages are in this category, out of 38 total. Annex 2 who good practices for pharmaceutical microbiology.
Glassware and instruments used in microbiology laboratory. Cleaning and validation of laboratory glassware lab. An indispensable tool in any microbiology laboratory is the inoculating loop. Microbiology laboratory is a basic laboratory in biology domain, to prepare a complete microbiology laboratory, do you know which are the microbiology laboratory essential instruments. A number of techniques are routine in microbiology laboratories that enable microorganisms to be cultured, examined and identified. Laboratory manual and workbook in microbiology applications to patient care 7th edition josephine a. This accuracy is known to the user through a few different pieces of information on the glassware. Microbial ecology deals with the interaction of microorganisms with one another and their biotic and abiotic environment. Skill in the logical communication of microbiology laboratory concepts through effective report writing and oral presentation.
Lab glassware are the essential parts of most experimental labs like chemistry, biochemistry, biology, genetics. In close partnership with the european commission and who, ecdc is currently exploring areas of work within its mandate that fall within the remit of this technical report on core functions of microbiology reference laboratories. Can it be determined that the plates were incubated for the correct length of time. Aim to get acquainted with the different glass wares and lab wares used in the microbiology laboratory. The beam has an exceptionally short wavelength, and increases the resolution of the image significantly over light microscopy. Other glassware is used to measure specific volumes, so it cant change its size appreciably over room temperatures. It has the same working principle as a domestic pressure cooker. Dont use the lab bench as a storage area for coats, books, etc. They are also required in large numbers as small amounts of reagents can be taken at a time.
We look forward to a rewarding and enlightening experience with you all. Safety in a microbiology laboratory is important in the prevention of infection as microbiology laboratory cultures, manipulates, and uses virulent andor potentially pathogenic microorganisms. Immediately report all accidents and spills to the instructor. Aim to study the working principle and operation of equipments used in microbiology laboratory. The components of the media are weighed in a given proportion, mixed in the required amount of water and sterilised in autoclave. They are mostly nongraduated as one can just add a desired volume from a pipette or burette. Determination of the cleaning efficiency for glassware in. They are used to heat on hold reagents for observing. Different chemicals and media used in a microbiology laboratory. Cleaning of glass ware for microbiology objective to lay down the procedure for cleaning of glass ware for microbiology. Laboratory equipment used in the microbiology laboratory should not be used outside the microbiology area, unless there are speci.
Emphasis on critical thinking and interpretation of test results. Degree in microbiology and either a board certification by the american board of microbiology and four years of clinical laboratory experience in microbiology, two of which must have been at the supervisory level, in an approved medical, hospital, or public health laboratory. Instruments used in microbiology are not fully automated 2. Since then, the number of requests for reprints on analytical methods used by the microbiology division, office of public health and science, food. Analysis results can be communicated with any degree of confidence only if the glassware used is free from contamination from previous analysis. Many of the ultraviolet uv sources used in the laboratory emit high intensities of uv light, capable of producing painful eye and skin burns. It is used for sterilization of glasswares, such as test tubes, pipettes and petri dishes.
Cleaning of glass ware for microbiology pharmaceutical. Overview of biochemical tests used to identify bacteria in. No need to wait for office hours or assignments to be graded to find out where you took a wrong turn. The growth of organisms that can metabolize mannose sugar will result in the agar turning yellow, while the agar color remains unchanged if the mannose is not metabolized. In most of the big labs, glassware is washed in three steps like. Adequate utilization of reference resources such as bergeys manual of systematic bacteriology. Internal quality control consists of all the procedures undertaken by a laboratory for the continuous evaluation of its work in order to ensure the consistency of results daytoday and their conformity with defined. Pdf automation in the clinical microbiology laboratory. The loop is a piece of wire that is looped at one end. Reading through posts here, it is obvious that things are done differently in the us than im used to, so please be patient.
Find 9780321994936 laboratory experiments in microbiology 11th edition by christine case et al at over 30 bookstores. Isbn 9780321994936 laboratory experiments in microbiology. Ankur choudhary print question forum no comments 1. Al228 suggestions for cleaning glassware 3 cleaning. Sop for cleaning and sterilization of glassware used in. Laboratory quality assurance according to isoiec17025 clause 5. Scope this sop is applicable for cleaning of glass ware in microbiology laboratory of pharmaceutical company name. These are the most commonly used glassware in the labs. Volumetric glassware, however, is considered very accurate.
Laminar flow bench is another most important instrument in any microbiology laboratory. Parenthetically, none of them allowed microbiologists to abandon the dogma of the pure culture technique, to escape the need to study microorganisms under the most unnatural conditions, or to work with the laboratory variants of. Validation of stated shelf life in microbiology lab. Introduction to the microbiology lab welcome to microbiology at miramar college. Some of these rules and procedures are listed in the. Some of the glassware instruments used in microbiology laboratory are. Determination of the cleaning efficiency for glassware in the pharmaceutical microbiology laboratory article pdf available in european journal of parenteral and pharmaceutical sciences 216. The use of classical culturedependent analysis of microbial communities reveals limited information about the microorganisms present. Is the media used able to be traced back to the date of preparation, the manufacturer, the sterility results etc.
Also learn about the process of handling and caring of laboratory glasswares. Chemicals may be heated and cooled so the glass needs to resist shattering from thermal shock. A laboratory manual is designed to meet the diverse requirements of upper division and graduatelevel laboratory sessions in environmental microbiology. It is used not only to sterilize liquid substances such as prepared media and saline diluents solutions, but also to sterilize glassware s, when required. Glassware funnel aids in pouring liquids into small openings without spilling them. If the glassware is dirty and cannot be washed immediately, put it in water to soak. Some of them, which are required in bulk quantity, are given below. Such dry sterilization is done only for glasswares. To help identify enterococcus and some clostridia which have ability to metabolize litmus milk. List of equipment apparatus used in microbiology laboratory.
The clinical microbiology laboratory has historically been considered lowtech, especially when compared to the clinical chemistry laboratory. Sterilization is widely employed to destroy all forms of living microorganism in a substance. The equipment is electrically powered and uses ammonia as the refrigerant. This training presentation provides information about.
Watch this video to learn more about the different types of lab glass. Up until a few years ago, out bacteriology lab was a 8 to 5. Laboratory glassware, how to clean and sterilization. Laboratory manual in general microbiology p a g e 6 5. A laboratory manual by jane ann phillips and a great selection of related books, art and collectibles available now at. Electron microscope is widely used for study of viruses and other microorganism at molecular level.
H7 outbreak in the pacific northwest focused national attention on food safety. Prepares and performs analyses on bacteria used for student unknowns to observe for mutation and effectiveness. Do not clean glassware if you are alone in the lab. Always clean your apparatus immediately after each use.
Care and safe handling of laboratory glasswar sigmaaldrich. Dispose of all contaminated materials in autoclave bags. Whole objects are coated in gold or palladium, which deflects the electron beam, creating dark and light areas as 3d images viewed on a monitor. Qa is associated with the three phases of quality assurance. Purpose to have a documented approved procedure that describes how to perform validation for stated shelf lives for prepared sterile media, poured agar plates, prepared filtered disinfectant, sterile glassware used in microbiology lab. The energy source used in the electron microscope is a beam of electrons. But glassware are more common due to time tested use and suitable for almost all the experiments. Cleanliness of laboratory glassware before use is of utmost importance.
Good laboratory technique demands clean glassware because the most carefully executed piece of work may give an erroneous result if dirty glassware is used. Microbiology laboratory safety rules and procedures each student enrolled in a microbiology laboratory at youngstown state university must follow specific safety rules and procedures. Laboratory ware and equipment that are not chemically clean are responsible for considerable losses in personnel time and supplies in many laboratories. Review of microbiology laboratory capacity, efficiency and. It is much easier to clean the glassware before residues in them become dry and hard. Basic instruments of any microbiology laboratories. D microbiology 7 introduction welcome to the microbiology laboratory. They are of different sizes, like 5ml, 10mk, 15ml etc. Instruments techniques and principals in microbiology 1. Glassware cleaning using clean glassware is key to the success of a chemical reaction, and cleaning glassware is part of good lab citizenship. Sop for cleaning of glassware used for microbiological. Most laboratory glassware is manufactured with borosilicate glass, a particularly durable glass that can safely be used to hold chemicals being heated over a flame and.
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